Computer assisted sperm analysis
Computer-aided sperm analysis CASA technology was developed in the late s for analyzing sperm movement characteristics or kinematics and has been highly successful in enabling this field of research. CASA has also been used with great success for measuring semen characteristics such as sperm concentration and proportions of progressive motility in many animal species, including wide application in domesticated animal production laboratories and reproductive toxicology. However, attempts to use CASA for human clinical semen analysis have largely met with poor success due to the inherent difficulties presented by many human semen samples caused by sperm clumping and heavy background debris that, until now, have precluded accurate digital image analysis. The authors review the improved capabilities of two modern CASA platforms Hamilton Thorne CASA-II and Microptic SCA6 and consider their current and future applications with particular reference to directing our focus towards using this technology to assess functional rather than simple descriptive characteristics of spermatozoa.
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The future of computer-aided sperm analysis Mortimer ST, Horst Gv, Mortimer D - Asian J Androl
The predictive value of sperm motility parameters obtained by computer-assisted semen analysis CASA was evaluated for the fertility of men from general population. In a prospective study with couples stopping use of contraception in order to try to conceive, CASA was performed on semen samples from men. This system has an editing function which allows correction of tracks made by the computer. Correlation between CASA parameters and fertility of normal couples measured as probability of achieving pregancy was examined by the Cox regression model.
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Computer-assisted sperm analysis (CASA): capabilities and potential developments.
Remarkably, basic concepts for identifying sperm and their motion patterns are little changed. Older and slower systems remain in use. Most major spermatology laboratories and semen processing facilities have a CASA system, but the extent of reliance thereon ranges widely. This review describes capabilities and limitations of present CASA technology used with boar, bull, and stallion sperm, followed by possible future developments. Each marketed system is different.
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